KAIST 생명과학과동창회
  • News & Events
  • News

News

November 19, 2020

Newly discovered metabolic pathway uses single carbon gases as a feedstock

by




18-newlydiscove.jpg


Figure 1. Acetyl-CoA production ratio in wild-type and knockout strains. Credit:  


A joint research team, affiliated with UNIST has identified a new metabolic pathway, in which microorganisms use single carbon (C1) gasses (CO and CO2) as a feedstock. The new metabolic pathway is thought to be the most energetically efficient pathway, compared to the existing ones, and thus is expected to be used in a variety of industrial applications that involved the conversion of C1 gas into value-added biochemicals.


Published in Proceedings of the National Academy of Sciences, this research has been jointly carried out by Professor Donghyuk Kim (School of Energy and Chemical Engineering, UNIST) and Professor Byung-Kwan Cho (Department of Biological Sciences, KAIST), joined by Dr. Yoseb Song (Department of Biological Sciences, KAIST) as the first author.

There are currently six autotrophic CO2 fixation pathways, capable of converting C1 gas into and one representative example is the photosynthesis in plants. Among those CO2-fixing in nature, the linear wood-ljungdahl (WLP) in phylogenetically diverse acetateforming acetogens is known to be the most energetically efficient pathway to fix C1 compounds. In particular, acetogens play an important role in the , with nearly 1,013 kg (100 billion US tons) of acetic acid being formed annually.


However, the growth rate of acetogens is 10 times slower than that of industrial microorganisms, such as E. coli. And this puts a limit on its use as industrial microorganisms for the conversion of C1 gas into useful biochemical products. Accordingly, many studies on a new and more effective CO2 fixation have been carried out.


19-newlydiscove.jpg

Figure 2. Construction of the genome-scale metabolic network model of C. drakei


The research team paid special attention to the growth rate of Clostridium drakei, which was faster than that of the other microorganisms, when accompanied by CO2 absorption. And, through this, they expected they might find clues to enhance the C1 gas conversion efficiency.


In this study, using the reconstructed genome-scale metabolic model iSL771 based on the completed genome sequence, transcriptomics, 13C isotope-based metabolite-tracing experiments, biochemical assays, and heterologous expression of the pathway in another acetogen, the research team discovered that the WLP and the glycine synthase pathway are functionally interconnected to fix CO2, subsequently converting CO2 into acetyl-CoA, acetyl-phosphate, and serine.


Moreover, the functional cooperation of the pathways enhances CO2 consumption and cellular growth rates via bypassing reducing power required reactions for cellular metabolism during autotrophic growth of acetogens.

"With the new CO2-fixing metabolic pathway, we shall overcome limitations in the biosynthesis for the production of high value-added compounds, brought by the slow growth rate of acetogens," says Professor Kim. 



https://phys.org/news/2020-11-newly-metabolic-pathway-carbon-gases.html


List of Articles
번호 제목 글쓴이 날짜 조회 수
139 김미영 교수, KAIST 이원조교수로 선정! 과사무실 2010.10.25 19140
138 김고운 박사과정생(조경옥 교수 Lab), 2010년 한국분자세포생물학회 우수 포스터상 수상 과사무실 2010.10.15 15415
137 김미영 교수, 박서영 박사과정생(Prof. Walton Jones Lab), '청암과학펠로' 선정 과사무실 2010.10.11 19544
136 학사과정 김혜림, 국립암센터 인턴쉽 포스터발표 최우수상 수상 과사무실 2010.09.07 13731
135 조홍석 박사과정 학생, Traveling Award 및 Best Presentation Award 수상 과사무실 2010.07.16 11775
134 이균민 교수, 교무처장 인사 발령 과사무실 2010.07.15 11933
133 임대식 교수, 세포분열시 MST1 kinase의 새로운 암 억제 기능 발견 (2010. 3) 과사무실 2010.07.09 11731
132 김대수 교수, PNAS에 논문 게재! 과사무실 2010.07.05 12564
131 임대식 교수, PNAS에 논문 게재! 과사무실 2010.04.21 12261
130 임대식 교수, 교과부 ' 창의적연구진흥사업'의 신규 지원과제에 선정! 과사무실 2010.04.21 12531
129 박태관 교수, 2010년도 삼성 고분자 학술상 수상! 과사무실 2010.04.12 10116
128 제1회 젊은 파스퇴르상 카이스트 석권! 과사무실 2010.03.02 11934
127 2010년 개교 39주년 기념 우수교원 포상 과사무실 2010.02.11 11645
126 허원도 교수, ECLIPSE 기술 개발 PNAS지 게재 과사무실 2010.02.09 13118
125 박태관 교수, 획기적인 핵산 유전자 치료제 전달시스템 개발 과사무실 2010.01.29 12479
124 학사과정 강병권 학생, 2009년 인성장학생 공로부문 선정! 과사무실 2010.01.08 12603
123 서연수 교수, 2009년 연구윤리 확립 유공자 교육과학기술부 장관 표창 수상! 과사무실 2010.01.06 12189
122 생명과학과 신임교원 강석조 박사 소개 (2010.01.01) 과사무실 2009.12.29 16415
121 BK21 생물사업단, 2단계 사업 1,2,3차년도 연차평가 생물분야 연속 1위! 과사무실 2009.12.29 13119
120 권석규 학생(김은준 교수 lab), 2009' 석림학술장학재단 장학생으로 선정! 과사무실 2009.12.22 13035
Board Pagination Prev 1 ... 11 12 13 14 15 16 17 18 19 20 ... 22 Next
/ 22