KAIST 생명과학과동창회
  • News & Events
  • News

News

우리 학과 송지준 교수와 제1저자 조수민 연구교수장주원 박사과정 학생이 nature communications에 “Structural basis of nucleosome assembly by the Abo1 AAA+ ATPase histone chaperone.”로 논문이 게재되었습니다.


(KI, E4, A426)송지준교수님.jpg




 2019 Dec 17;10(1):5764. doi: 10.1038/s41467-019-13743-9.

Structural basis of nucleosome assembly by the Abo1 AAA+ ATPase histone chaperone.

Cho C1Jang J2Kang Y3Watanabe H4,5Uchihashi T5,6Kim SJ7Kato K4,5,8Lee JY9Song JJ10.

Abstract

The fundamental unit of chromatin, the nucleosome, is an intricate structure that requires histone chaperones for assembly. ATAD2 AAA+ ATPases are a family of histone chaperones that regulate nucleosome density and chromatin dynamics. Here, we demonstrate that the fission yeast ATAD2 homolog, Abo1, deposits histone H3-H4 onto DNA in an ATP-hydrolysis-dependent manner by in vitro reconstitution and single-tethered DNA curtain assays. We present cryo-EM structures of an ATAD2 family ATPase to atomic resolution in three different nucleotide states, revealing unique structural features required for histone loading on DNA, and directly visualize the transitions of Abo1 from an asymmetric spiral (ATP-state) to a symmetric ring (ADP- and apo-states) using high-speed atomic force microscopy (HS-AFM). Furthermore, we find that the acidic pore of ATP-Abo1 binds a peptide substrate which is suggestive of a histone tail. Based on these results, we propose a model whereby Abo1 facilitates H3-H4 loading by utilizing ATP.


41467_2019_13743_Fig7_HTML.jpg


Abo1 binds the H3 tail at the central pore to facilitate histone deposition.

a Extra density near the Abo1 central pore region, surrounded by Abo1 W345 tryptophan side chains. b Polyalanine model of the substrate peptide (magenta) built into the extra electron density observed in a, with W345 side chains colored in yellow. c Superposition of Abo1 AAA1 pore loops 1 & 2 with other AAA+ ATPases. Abo1 (dark blue), Hsp104 in the extended state, (gray, PDB ID: 5VYA), p97 in the ATPγS state (light green, PDB ID: 5FTL), and NSF in the ATP state (pink, PDB ID: 3J94) were aligned by AAA1 NBD. Key substrate binding residues on pore loop 1 and the acidic residue of pore loop2, E385, are labeled. d Electrostatic surface representation of Abo1 highlighting the negatively charged pore interior contributed by acidic pore loop residues. e Depiction of intermolecular crosslinks between Abo1 and histone H3–H4 detected by XL-MS and filtered with a crosslink confidence (LD) score cutoff of 20. The highest LD score intermolecular crosslink, a crosslink between H3 the N-terminus (K4) and Abo1 AAA1 pore loop1 (K344), is labeled. f DNA curtain-based H3–H4 deposition assays performed with Abo1 pore loop mutants (W345A and E385A) and a bromodomain mutant (E900A) with wild-type H3–H4, and H3–H4 deposition assays of wild-type Abo1 with N-terminal tail truncated H3–H4. g Comparison of H3–H4 loading activity on DNA by quantification of fraction DNA bound with labeled H3–H4. Error bars represent SD from three experiments. The numbers of molecules analyzed for each condition are n = 244 for WT, n = 222 for W345A, n = 219 for E385, n = 249 for E900A and n = 238 for tailless H3–H4.



https://www.ncbi.nlm.nih.gov/pubmed/31848341


Cho_Jang-NatureCom (1).pdf

List of Articles
번호 제목 글쓴이 날짜 조회 수
138 송세라, 박영균 학생 김보정 기초과학장학생으로 선발 과사무실 2006.09.12 12292
137 송지준 교수, Genes & Dev. 게재 (2013.3) 과사무실 2013.03.21 12472
136 송지준 교수, J.Am.Chem.Soc. 게재 (2013.10) 과사무실 2013.10.18 12275
135 송지준 교수, PNAS 게재 (2012.8) 과사무실 2012.12.10 12762
134 송지준 교수, 김은지 박사 Molecular Cell에 논문 게재(2015) / Prof. Ji-Joon Song and PhD. Eun-Ji Kim published a paper at Molecular Cell (2015) 과사무실 2015.07.29 14043
» 송지준교수, 조수민 연구교수, 장주원 박사과정학생 nature communications 논문 게재 file 생명과학과 2020.01.02 21688
132 오병하 교수, 제 9회 아산의학상 수상 file 생명과학과 2016.03.14 17178
131 오병하 교수님_자연에 없는 고감도 단백질 센서 제작 플랫폼 개발 생명과학과 2021.02.08 502
130 우주연 학생(김은준 교수님 Lab), 로레알-유네스코 펠로쉽 수상 과사무실 2009.06.23 15438
129 윤기준 교수, '2019 서경배과학재단 신진 과학자' 선정 file 생명과학과 2019.09.23 5355
128 이규리 학생(박태관 교수님 Lab), ICMAT 2009 & IUMRS-ICA 2009 Best Poster Award 수상 과사무실 2009.07.13 12540
127 이균민 교수 생명과학과 학과장 취임 과사무실 2007.09.13 12282
126 이균민 교수, 교무처장 인사 발령 과사무실 2010.07.15 11454
125 이상기 박사 한국생명공학연구원장 취임 과사무실 2005.07.12 12573
124 이상열 교수(박사 85) KAIST 올해의 동문 선정 과사무실 2005.01.04 14167
123 이승재 교수, 올리브유의 노화 방지 및 장수 효과 입증 file 생명과학과 2019.08.22 5248
122 이승재 교수, 한성과학상 수상 file 생명과학과 2019.07.01 5868
121 이승재 교수님_One More Key to Human Longevity Found After Worm Research 생명과학과 2020.07.03 3757
120 이승희 교수, 송유향, 김재현 박사과정 학생 Neuron 게재 file 생명과학과 2017.02.13 17313
119 이승희 교수님_KAIST, 뇌 인지기능 높이는 물질 발견… 치매 치료 효과 기대 file 생명과학과 2020.04.26 3406
Board Pagination Prev 1 ... 6 7 8 9 10 11 12 13 14 15 ... 17 Next
/ 17