1. 연사 : 최의주 교수 (고려대)
2. 연제 : CIIA: a novel anti-apoptotic protein that antagonizes both ASK1 and CAD activities.
3. 일시 : 2004년 5월 11일 (화) 오후 4:00
4. 장소 : 의과학센터 원격강의실
※ 문의 : 정종경 교수님 (2620)
※ Abstract
Diverse stimuli initiate the activation of apoptotic signaling pathways that often causes nuclear DNA fragmentation. Here we report a new anti-apoptotic protein, CIIA (a CAD inhibitor that interacts with ASK1). CIIA, by binding to apoptosis signal-regulating kinase 1 (ASK1), inhibits oligomerization-induced ASK1 activation. CIIA also associates with caspase-activated DNase (CAD) and inhibits the nuclease activity of CAD without affecting caspase-3-mediated ICAD cleavage. Overexpressed CIIA reduces H2O2- and TNF-α-induced apoptosis. CIIA antisense oligonucleotides, which abolish expression of endogenous CIIA in murine L929 cells, block the inhibitory effect of CIIA on ASK1 activation, DNA fragmentation, and apoptosis. These findings suggest that CIIA is an endogenous antagonist of both ASK1-and CAD-mediated signaling.
2. 연제 : CIIA: a novel anti-apoptotic protein that antagonizes both ASK1 and CAD activities.
3. 일시 : 2004년 5월 11일 (화) 오후 4:00
4. 장소 : 의과학센터 원격강의실
※ 문의 : 정종경 교수님 (2620)
※ Abstract
Diverse stimuli initiate the activation of apoptotic signaling pathways that often causes nuclear DNA fragmentation. Here we report a new anti-apoptotic protein, CIIA (a CAD inhibitor that interacts with ASK1). CIIA, by binding to apoptosis signal-regulating kinase 1 (ASK1), inhibits oligomerization-induced ASK1 activation. CIIA also associates with caspase-activated DNase (CAD) and inhibits the nuclease activity of CAD without affecting caspase-3-mediated ICAD cleavage. Overexpressed CIIA reduces H2O2- and TNF-α-induced apoptosis. CIIA antisense oligonucleotides, which abolish expression of endogenous CIIA in murine L929 cells, block the inhibitory effect of CIIA on ASK1 activation, DNA fragmentation, and apoptosis. These findings suggest that CIIA is an endogenous antagonist of both ASK1-and CAD-mediated signaling.
