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Abstract

The lateral amygdala (LA) has been proposed as a critical site for fear memory storage and, long-term potentiation (LTP) at LA synapses induced by fear conditioning has been thought to be the cellular mechanism underlying fear memory formation. However, similar synaptic changes are also observed in upstream areas, such as the medial geniculate nucleus (MGN) and auditory cortex (AC), raising the critical question of where the fear memory is actually formed and stored. Here we used optogenetic approach to determine if direct stimulation of the presynaptic auditory projections in the LA can serve as a conditioned stimulus (CS) that, when paired with a foot shock unconditioned stimulus (US), can drive fear conditioning. Mice were injected with AAV-ChR2 in the two main auditory pathways to LA, MGN and AC, sending auditory information into the LA during auditory fear conditioning. Optical photostimulation was used to activate ChR2-expressing presynaptic axons in the LA as a CS for fear conditioning. 24 hours later, the same optogenetic CS produced robust freezing responses while control mice injected with AAV-GFP did not. In parallel, we also examined LTP induction in LA slices with ChR2 expression in MGN and AC projections to LA neurons. Whole-cell patch recording showed that pairing photo-stimulation (3 Hz) of auditory projections in LA with postsynaptic current injection produced strong LTP at LA synapses. Our data provide compelling evidence that fear memory can be formed in the LA synapses independent of the activity of upstream areas delivering auditory information and LTP at the LA synapses may underlie fear memory formation.


  1. 학과설명회(11.21)

  2. 학부생 가을나들이(11.4~11.5)

  3. 추계 대학원생체육대회(11.2)

  4. 2017 가을학기 생명과학기술대학 Dean's List 시상식

  5. The 3rd EPFL-KAIST Symposium on Life Science and Bioengineering

  6. Bio URP 수료식(08.04)

  7. 2017. 5. 22 총장 생명과학과 방문

  8. 2017 실험동물위령제

  9. [2017 태울석림제] 생명과학과 축제 부스 운영

  10. University of Adelaide 학부생, KAIST 생명과학과 방문

  11. 2017 생명과학과 교수연수회

  12. [CEO포럼]삼성바이오에피스 민호성 상무 초청강연

  13. 2017 생명과학기술대학 Dean's List 시상식

  14. 3회 엔지노믹스 우수박사학위 논문상 시상식

  15. 2기 Medytox Fellowship 장학증서 수여식(2.27)

  16. 강창원 교수님 정년퇴임 기념 강연(2.20)

  17. 제11회 BK21 플러스 생물사업단 대학원생 연구발표회(2.20~2.22)

  18. 학부졸업생 시상(최우등/ 리더십, 봉사 우수/생화학분자생물학회장상)

  19. 2017 Hooding Ceremony(2.17)

  20. 2016 생명과학과 학과설명회

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