KAIST 생명과학과동창회
  • News & Events
  • News

News

우리 학과 송지준 교수와 제1저자 조수민 연구교수장주원 박사과정 학생이 nature communications에 “Structural basis of nucleosome assembly by the Abo1 AAA+ ATPase histone chaperone.”로 논문이 게재되었습니다.


(KI, E4, A426)송지준교수님.jpg




 2019 Dec 17;10(1):5764. doi: 10.1038/s41467-019-13743-9.

Structural basis of nucleosome assembly by the Abo1 AAA+ ATPase histone chaperone.

Cho C1Jang J2Kang Y3Watanabe H4,5Uchihashi T5,6Kim SJ7Kato K4,5,8Lee JY9Song JJ10.

Abstract

The fundamental unit of chromatin, the nucleosome, is an intricate structure that requires histone chaperones for assembly. ATAD2 AAA+ ATPases are a family of histone chaperones that regulate nucleosome density and chromatin dynamics. Here, we demonstrate that the fission yeast ATAD2 homolog, Abo1, deposits histone H3-H4 onto DNA in an ATP-hydrolysis-dependent manner by in vitro reconstitution and single-tethered DNA curtain assays. We present cryo-EM structures of an ATAD2 family ATPase to atomic resolution in three different nucleotide states, revealing unique structural features required for histone loading on DNA, and directly visualize the transitions of Abo1 from an asymmetric spiral (ATP-state) to a symmetric ring (ADP- and apo-states) using high-speed atomic force microscopy (HS-AFM). Furthermore, we find that the acidic pore of ATP-Abo1 binds a peptide substrate which is suggestive of a histone tail. Based on these results, we propose a model whereby Abo1 facilitates H3-H4 loading by utilizing ATP.


41467_2019_13743_Fig7_HTML.jpg


Abo1 binds the H3 tail at the central pore to facilitate histone deposition.

a Extra density near the Abo1 central pore region, surrounded by Abo1 W345 tryptophan side chains. b Polyalanine model of the substrate peptide (magenta) built into the extra electron density observed in a, with W345 side chains colored in yellow. c Superposition of Abo1 AAA1 pore loops 1 & 2 with other AAA+ ATPases. Abo1 (dark blue), Hsp104 in the extended state, (gray, PDB ID: 5VYA), p97 in the ATPγS state (light green, PDB ID: 5FTL), and NSF in the ATP state (pink, PDB ID: 3J94) were aligned by AAA1 NBD. Key substrate binding residues on pore loop 1 and the acidic residue of pore loop2, E385, are labeled. d Electrostatic surface representation of Abo1 highlighting the negatively charged pore interior contributed by acidic pore loop residues. e Depiction of intermolecular crosslinks between Abo1 and histone H3–H4 detected by XL-MS and filtered with a crosslink confidence (LD) score cutoff of 20. The highest LD score intermolecular crosslink, a crosslink between H3 the N-terminus (K4) and Abo1 AAA1 pore loop1 (K344), is labeled. f DNA curtain-based H3–H4 deposition assays performed with Abo1 pore loop mutants (W345A and E385A) and a bromodomain mutant (E900A) with wild-type H3–H4, and H3–H4 deposition assays of wild-type Abo1 with N-terminal tail truncated H3–H4. g Comparison of H3–H4 loading activity on DNA by quantification of fraction DNA bound with labeled H3–H4. Error bars represent SD from three experiments. The numbers of molecules analyzed for each condition are n = 244 for WT, n = 222 for W345A, n = 219 for E385, n = 249 for E900A and n = 238 for tailless H3–H4.



https://www.ncbi.nlm.nih.gov/pubmed/31848341


Cho_Jang-NatureCom (1).pdf

List of Articles
번호 제목 글쓴이 날짜 조회 수
330 임대식 최길주 교수 승진 인사발령 과사무실 2004.09.01 14539
329 임대식 교수님 실험실 (김민철 박사과정) The Embo Journal 게재(2013.5) 과사무실 2013.07.05 15263
328 임대식 교수, 한국과학상 대통령상 수상(2016. 12. 27) file 생명과학과 2016.12.28 13182
327 임대식 교수, 천주교 서울대교구 생명위원회 제 14회 '생명의 신비상' 수상 file 생명과학과 2019.12.26 4935
326 임대식 교수, 세포분열시 MST1 kinase의 새로운 암 억제 기능 발견 (2010. 3) 과사무실 2010.07.09 11591
325 임대식 교수, 김민철 박사 Cell Reports 에 논문 게재(2015.03) / Professor Dae-Sik Lim and Min Cheol Kim, Ph.D Publish in Cell Reports (2015.03) 과사무실 2015.04.06 14453
324 임대식 교수, 교과부 ' 창의적연구진흥사업'의 신규 지원과제에 선정! 과사무실 2010.04.21 12366
323 임대식 교수, 과학기술혁신본부장에 임명 file 생명과학과 2017.09.01 15082
322 임대식 교수, PNAS에 논문 게재! 과사무실 2010.04.21 12088
321 임대식 교수, PNAS 게재 (2013.4) 과사무실 2013.05.02 12574
320 임대식 교수, KAIST 지정 석좌교수 임용 과사무실 2014.02.25 11970
319 임대식 교수, EMBO Journal 에 논문 게재 (2012.3.7) 과사무실 2012.04.17 12687
318 임대식 교수, 2016년도 한국분자세포생물학회 정기학술대회 학술상 생명과학상 소개 강연 생명과학과 2016.10.10 13645
317 임대식 교수 외 3명 개교 34주년 기념 우수교원 포상 과사무실 2005.02.15 10868
316 임대식 교수 논문 Nature Cell Biology 게재 1 과사무실 2004.01.26 13650
315 임대식 교수 국가지정연구실사업 신규과제 선정 과사무실 2005.03.30 11447
314 인선아 박사과정 학생. 제 18차 KHUPO 프로테오믹스 국제학술대회 수상 file 생명과학과 2018.04.13 12771
313 이중재 박사와 김학성 교수 연구팀, Theranostics에 표지 논문 발표 file 생명과학과 2017.08.18 12643
312 이준식 교수 IAFOST Fellow 선출 과사무실 2003.09.08 13390
311 이주용 박사, 김세윤 교수, PNAS지에 논문 게재 / PhD Joo-Yong Lee and Prof, Seyun Kim published a paper in PNAS 생명과학과 2016.07.04 16339
Board Pagination Prev 1 2 3 4 5 6 7 8 9 10 ... 22 Next
/ 22