허원도 교수, 김진만 박사, 이민지 박사과정 학생 PNAS지에 논문 게재(2016.05) / Prof. Won-Do Heo, PhD. Jin-Man Kim and Min-Ji lee publish an article in PNAS (2016.05)
Optogenetic toolkit reveals the role of Ca2+ sparklets in coordinated cell migration.
In this article, we present a pioneering experimental approach for studying cell migration and more broadly establish representative guidelines for applying an optogenetic approach in biological studies. Using recently developed optogenetic tools, we identified local Ca2+ influx as a major source of Ca2+ for gradient formation and established the functional importance of polarized chemistry in highly coordinated cell migration. These findings provide strong evidence for a mechanism that addresses fundamental questions about front–rear Ca2+ gradients in migrating cells and suggest a previously unidentified role of voltage-dependent Ca2+ channels in the directional migration of nonexcitable cells.